Skip to main content

Advertisement

Log in

A Study on Detection of Pathogenic Listeria monocytogenes in Ovine’s of Kashmir Region Having Abortion or History of Abortion

  • Research Article
  • Published:
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The present investigation delineates the role of listeric infection in aborted ewes or those with history of abortion from organized farms of Kashmir region. A total of 141 clinical samples was analyzed for the isolation and identification of Listeria species. On analysis four isolates were identified as Listeria monocytogenes, while 24 were non-pathogenic Listeria species. Of the four L. monocytogenes isolates, two were isolated from brain tissue of aborted fetus (BrS10 and BrG36) while one each was isolated from vaginal swab (Vd13) and rectal swab (RS11) of the ewe. An overall isolation rate of 2.83 % was observed for L. monocytogenes and 17.02 % for non-pathogenic Listeria species. Further to reveal the pathogenic potential, the recovered L. monocytogenes isolates were subjected to the battery of in vitro pathogenicity test such as hemolytic activity on Sheep Blood Agar, Phosphatidyl Inositol-Phospholipase C activity on Agar Listeria according to Ottaviani and Agosti medium, multiplex PCR targeting virulence markers genes viz., prfA, plcA, actA, hly, inlC and in vivo chick embryo inoculation test. All the L. monocytogenes isolates recovered in the present study were potentially pathogenic and on comparison, a good correlation was observed among in vitro and in vivo pathogenicity test including multiplex PCR targeting virulence associated genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Barbuddhe SB, Chakraborty T (2009) Listeria as an enteroinvasive gastrointestinal pathogen. Curr Top Microbiol Immunol 337:173–195

    PubMed  CAS  Google Scholar 

  2. Low JC, Donachie W (1997) A review of Listeria monocytogenes and listeriosis. Vet J 153:9–29

    Article  PubMed  CAS  Google Scholar 

  3. Liu D (2006) Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen. J Clin Microbiol 55:646–659

    Google Scholar 

  4. Vishwanathan GR, Ayyar VV (1950) Circling disease of sheep in the Madras state-etiology established. Indian Vet J 26:395

    Google Scholar 

  5. Sharma M, Batta MK, Katoch RC (1996) Listeria monocytogenes abortion among migratory sheep and goats in Himachal Pradesh. Indian J Anim Sci 66:1117–1119

    Google Scholar 

  6. Mahajan AK, Katoch RC (1997) Aerobic microbiol flora associated with endometritis in sheep and goats. Indian J Anim Sci 67:290–291

    Google Scholar 

  7. Chand P, Sadana JR (1999) Outbreak of Listeria ivanovii abortion in sheep in India. Vet Rec 145:83–84

    Article  PubMed  CAS  Google Scholar 

  8. Kumar H, Singh BB, Kaur KB, Singh R, Sidhu PK, Sandhu KS (2007) Pathological and epidemiological investigations into listerial encephalitis in sheep. Small Rumin Res 71:293–297

    Article  Google Scholar 

  9. Rocourt J, Schrettenbrunner A, Seeliger HPR (1983) Differenciation biochimique des groupes genomiques de Listeria monocytogenes (sensu lato). Ann Microbiol 134A:65–71

    CAS  Google Scholar 

  10. Paziak DB, Bogusławska E, Wieckowska SM, Kotłowski R, Rózalska B, Chmiela M, Kur J, Dabrowski W, Rudnicka W (1999) Evaluation of the API test, phosphatidylinositol-specific phospholipase C activity and PCR method in identification of Listeria monocytogenes in meat foods. FEMS Microbiol Lett 171:209–211

    Article  Google Scholar 

  11. Shakuntala I, Malik SVS, Barbuddhe SB, Rawool DB (2006) Isolation of Isolation of Listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction. Vet Microbiol 117:229–234

    Article  PubMed  CAS  Google Scholar 

  12. Kaur S, Malik SVS, Vaidya VM, Barbuddhe SB (2007) Listeria monocytogenes in spontaneous abortions in humans and its detection by multiplex PCR. J Appl Microbiol 27:1889–1896

    Article  CAS  Google Scholar 

  13. Rawool DB, Malik SVS, Barbuddhe SB, Shakuntala I, Aurora R (2007) A multiplex PCR for detection of virulence associated genes in Listeria monocytogenes. Intl J Food Saf 9:56–62

    Google Scholar 

  14. Barbuddhe SB, Malik SVS (2009) Recent advances in the molecular diagnosis of Listeriosis. In: ICAR sponsored winter school on “Molecular diagnostic techniques for zoonotic and foodborne infections”, Feb 07–27, Division of Veterinary Public Health, Indian Veterinary Research Institute, Izatnagar, pp. 191–199

  15. Kaur S, Malik SVS, Bhilegaonkar KN, Vaidya VM, Barbuddhe SB (2009) Use of a phospholipase-C assay, in vivo pathogenicity assays and PCR in assessing the virulence of Listeria spp. Vet J 184:366–370

    Article  PubMed  CAS  Google Scholar 

  16. McClain D, Lee WH (1988) Development of USDA-FSIS method for isolation of Listeria monocytogenes from raw meat and poultry. J Assoc Off Anal Chem 71:660–664

    PubMed  CAS  Google Scholar 

  17. Notermans S, Dufrenne J, Chakraborty T, Steinmeyer S, Terplant G (1991) The chick embryo test agrees with the mouse bio-assay for assessment of the pathogenicity of Listeria species. Lett Appl Microbiol 13:161–164

    Article  Google Scholar 

  18. Dhanda MR, Lall JM, Seth RN, Chandrasekhariah P (1959) A case of listeric abortion in an ewe with a small scale survey of the incidence of agglutinins of Listeria in the sera of sheep. Indian Vet J 36:113–124

    Google Scholar 

  19. Sharef J (2009) Studies on prevalence of Listeria spp. in sheep of Kashmir valley. Dissertation, SKUAST-Kashmir

  20. Nigam P, Katoch RC, Sharma M, Verma S (1999) Investigation on listeriosis associated with reproductive disorders of domestic animals in Himachal Pradesh. Indian J Anim Sci 69:171–173

    Google Scholar 

  21. Kampelmacher EH, van Noorle Jansen LM (1969) Isolation of Listeria monocytogenes from faeces of clinically healthy humans and animals. Zentralbl Bakteriol Orig 211(3):353–359

    PubMed  CAS  Google Scholar 

  22. Anonymous (1991) Listeria monocytogenes. Recommendation by the National Advisory Committe on Microbiological Criteria for Foods. Inst J Food Microbiol 14:185

    Google Scholar 

  23. Farber JM, Peterkin PI (1991) Listeria monocytogenes, a food borne pathogen. Microbiol Rev 55:476–511

    PubMed Central  PubMed  CAS  Google Scholar 

  24. Vazquez-Boland JA, Kuhn M, Berche P, Chakraborty T, Domı′nguez-Bernal G, Goebel W, Gonza′lez-Zorn B, Wehland J, Kreft J (2001) Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev 14:584–640

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  25. Courteiu AL (1991) Latest news on Listeriosis. Comp Immunol Microbiol Infect Dis 14:1–7

    Article  Google Scholar 

  26. Rawool DB, Malik SVS, Shakuntala I, Sahare AM, Barbuddhe SB (2007) Detection of multiple virulence associated genes in pathogenic Listeria monocytogenes from bovines with mastitis. Int J Food Microbiol 113:201–207

    Article  PubMed  CAS  Google Scholar 

  27. Aurora R, Prakash A, Prakash S, Rawool DB, Barbuddhe SB (2008) Comparison of PI-PLC based assays and PCR along with in vivo pathogenicity tests for rapid detection of pathogenic Listeria monocytogenes. Food Control 19:641–647

    Article  CAS  Google Scholar 

  28. Ottaviani F, Ottaviani M, Agosti M (1997) Esperienza su um agar selettivo e differentiale per Listeria monocytogenes. Industrie Alimentari 36:1–3

    Google Scholar 

  29. Nishibori T, Cooray K, Xiang H, Kawmuru I, Fujita M, Mitsuyama M (1995) Correlation between the presence of virulence associated genes as determined by PCR and actual virulence in mice in various strains of Listeria spp. Micobiol Immunol 39:343–349

    Article  CAS  Google Scholar 

  30. Shivaramu KV (2008) Studies on listeric infections in sheep by cultural, serological and molecular methods. Dissertation, Indian Veterinary Research Institute, Izatnagar, Bareilly

  31. Chakraborty J, Goebel W (1988) Recent developments in the study of virulence in Listeria monocytogenes. Curr Top Microbiol Immunol 138:41–58

    PubMed  CAS  Google Scholar 

  32. Tabouret M, DeRycke J, Audurier A, Poutrel B (1991) Pathogenicity of Listeria monocytogenes isolates in immunocompromised mice in relation to production of listeriolysin. J Med Microbiol 34:13–18

    Article  PubMed  CAS  Google Scholar 

  33. Barbuddhe SB, Malik SVS, Kumar P (1999) High seropositivity against listeriolysin O in humans. Ann Trop Med Parasitol 93(5):537–539

    Article  PubMed  CAS  Google Scholar 

  34. Barbuddhe SB, Malik SVS, Bhilegaonkar KN, Kumar P, Gupta LK (2000) Isolation of Listeria monocytogenes and anti-listeriolysin-O detection in sheep and goats. Small Rum Res 38:151–155

    Article  Google Scholar 

  35. Chaudhari SP, Malik SVS, Banu RG, Barbuddhe SB (2001) Detection of anti-listeriolysin O and Listeria monocytogenes in experimentally infected buffaloes (Bubalus bubalis). Trop Anim Health Prod 33:285–293

    Article  PubMed  CAS  Google Scholar 

  36. Bhanu RV, Malik SVS, Chaudhari SP, Barbuddhe SB (2006) Listeriolysin O-based diagnosis of Listeria monocytogenes infection in experimentally and naturally infected goats. Small Rum Res 66:70–75

    Article  Google Scholar 

  37. Kalorey DR, Kurkure NV, Warke SR, Rawool DB, Malik SVS, Barbuddhe SB (2006) Isolation of pathogenic Listeria monocytogenes in faeces of wild animals in captivity. Comp Microbiol Immunol Infect Dis 29:295–300

    Article  CAS  Google Scholar 

  38. Notermans SHW, Dufrenne J, Leimeister-Wachter M, Domann E, Chakraborty T (1991) Phosphatidylinositol-specific phospholipase C activity as a marker to distinguish between pathogenic and non-pathogenic Listeria species. Appl Environ Microbiol 57:2666–2670

    Google Scholar 

  39. Suarez M, Vazquez-Boland JA (2001) The bacterial actin nucleter protein ActA is involved in epithelial cell invasion by Listeria monocytogenes. PUBMED [Accession No. AF103807]

  40. Liu D, Lawrence ML, Austin FW, Ainsworth AJ (2007) A multiplex PCR for species and virulence-specific determination of Listeria monocytogenes. J Microbiol Methods 71:133–140

    Google Scholar 

Download references

Acknowledgments

The authors thank Director, Indian Veterinary Research Institute, Izatnagar for providing necessary facilities to undertake the research. The work supported by Junior Research Fellowship to SS by Indian Council of Agricultural Research, New Delhi is duly acknowledged. The authors are grateful to Dr. M. M. Willayat, Head of VPH, FVSc and AH, Shuhama for extending his help during sample collection. They thank Mr. K. K. Bhat for excellent technical assistance.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Shabu Shoukat.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shoukat, S., Malik, S.V.S., Rawool, D.B. et al. A Study on Detection of Pathogenic Listeria monocytogenes in Ovine’s of Kashmir Region Having Abortion or History of Abortion. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 84, 311–316 (2014). https://doi.org/10.1007/s40011-013-0228-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40011-013-0228-0

Keywords

Navigation